Media for freezing homogenised faecal samples for re-cultivation
Page last modified on: 2024, November 06
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Metadata
| Title | Adapated and used by | last accessed | Link to protocol | Primary origin for protocol |
|---|---|---|---|---|
| Media for freezing homogenised faecal samples for re-cultivation / FMT | University Hospital of RWTH Aachen, Inst. Med. Microbiology, Clavel lab Collaborative Research Center 1382, Project Q02 “Integrative Microbiota Analysis”, Dr. Nicole Treichel | 2024-09-12 | (Treichel, 2022) | (“A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation.,” 2019) |
Protocol
- Make normal saline: 9.0 g/L NaCl
- Dissolve Maltodextrin and Trehalose in normal saline at 40 °C, to respective final concentrations of 18.75% and 6.25% (w/v; 1% = 1 g/100 mL). Add 0.5% (w/v) Ascorbic acid and 0.05% L-Cystein (w/v).
For 100 mL:
| amount | ingredient |
|---|---|
| 18.75 g | Maltodextrin |
| 6.25 g | Trehalose |
| 0.5 g | Ascorbic acid |
| 0.05 g | L-Cystein |
Material
- Maltodextrin PubChem SID: 481109074
- Trehalose
- L-Cystein
- Ascorbic acid
- NaCl
- Water (MQ/DEPC)
References
- Treichel, N. (2022). Media for freezing homogenised faecal samples for re-cultivation / FMT. CRC1382. https://www.crc1382.org/wp-content/uploads/2022/11/221019_FMT-Media-Protocol.pdf
- A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation. (2019). Scientific Reports, 9. https://doi.org/10.1038/s41598-019-45173-4