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Media for freezing homogenised faecal samples for re-cultivation




Page last modified on: 2024, December 12

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Title Adapated and used by last accessed Link to protocol Primary origin for protocol
Media for freezing homogenised faecal samples for re-cultivation / FMT University Hospital of RWTH Aachen, Inst. Med. Microbiology, Clavel lab Collaborative Research Center 1382, Project Q02 “Integrative Microbiota Analysis”, Dr. Nicole Treichel 2024-09-12 (Treichel, 2022) (“A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation.,” 2019)

Protocol


  1. Make normal saline: 9.0 g/L NaCl
  2. Dissolve Maltodextrin and Trehalose in normal saline at 40 °C, to respective final concentrations of 18.75% and 6.25% (w/v; 1% = 1 g/100 mL). Add 0.5% (w/v) Ascorbic acid and 0.05% L-Cystein (w/v).

For 100 mL:

amount ingredient
18.75 g Maltodextrin
6.25 g Trehalose
0.5 g Ascorbic acid
0.05 g L-Cystein

Material

References


  1. Treichel, N. (2022). Media for freezing homogenised faecal samples for re-cultivation / FMT. CRC1382. https://www.crc1382.org/wp-content/uploads/2022/11/221019_FMT-Media-Protocol.pdf
  2. A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation. (2019). Scientific Reports, 9. https://doi.org/10.1038/s41598-019-45173-4